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Molecular imaging reveals ONC201 antitumor efficacy and immune stimulation in cancer models
August 15 @ 11:00 am - 12:00 pm EDT
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ONC201 is a novel small molecule which is in clinical trials for both solid and hematopoietic malignancies.
To maximize the observed clinical benefits of ONC201 and further elucidate its mechanism of action, preclinical studies were developed to test the hypothesis that dose intensification of ONC201 may impact antitumor efficacy, inhibit metastasis, and promote an intra-tumoral immune response (cfr. Wagner et al. 2018 JCI). To properly investigate this hypothesis, multiple mouse models were used in conjunction with various in vivo imaging techniques including: microCT imaging to monitor tumour-burden within the lungs, MRI, and bioluminescence and fluorescence imaging using the IVIS® platform to track subcutaneous, orthotopic and metastatic tumor growth.
To further elucidate the immune-stimulatory and anti-metastatic potency of ONC201, confocoal microscopy of 2D co-culture in established human NK and tumor cells using Vectra® Automated Quantitative pathology imaging and Inform® Software of IHC xenograft sections were performed.
During this webcast, the speakers will address the benefits of cell culture, xenograft tissue sections and mouse models coupled with imaging in preclinical immuno-oncology trials.
During this webcast you will learn/our speaker(s) will address:
- The best choice of immunocompetent and immune-compromised animal tumor models to study cytotoxic, anti-metastatic effect and potential immunomodulatory effects of novel therapeutics;
- The utility of various in vivo imaging modalities and reporters for monitoring therapeutic response in subcutaneous, orthotopic and metastatic tumor models in immunocompetent and immunodeficient mice.
- The use of CT image sets to segment lung airspace and estimate tumor burden in a mouse model of lung cancer.
- A method for high throughput, high accuracy entire slide scanning of nuclear and cytosolic IHC stained mouse tumor tissues to monitor e.g. proliferation, apoptosis and neovascularization.
- Enhanced programming of automated quantitative pathology Inform software to avoid any necrotic, stromal, or glass portions of images.